The microbially induced calcium carbonate precipitation technique is used to grout cracks in rock mass,which has the advantages of low slurry viscosity,good grouting and green environmental protection.The field extrac-tion,activation culture and expansion culture of microbial strains were carried out,and the optimum conditions of mi-crobial bacterial culture were discussed.The microbial grout which could be used in field grout was prepared,and the field test of microbial grout in rock crack grouting was carried out according to the distribution characteristics of rock crack in field.The results showed that the initial viscosity of the slurry prepared by the local strains was 2 mPa·s～3 mPa·s,and it had good grouting.The microbial grouting construction technology,which is mainly characterized by repeated multiple perfusion and each quantitative perfusion,can be applied to the grouting of small cracks in rock mass,and the diffusion distance can reach more than 0.4 m,and the permeability of rock mass after grouting can reach the order of 0.1 Lu,with remarkable anti-seepage effect,providing a new technical means for the anti-seepage reinforcement treatment of rock mass cracks with an opening of less than 0.2 mm.
关键词
岩体/微小裂隙/MICP技术/微生物浆液/灌浆
Key words
rock mass/microcrack/MICP technology/microbial slurry/grouting
维普
万方数据