In order to analyze the effects of bacterial-binder ratio on the curing effects of microorganisms,sporosarcina pasteurii is used.Three groups of experiment corresponding to different bacterial-binder ratios are conducted to rein-force clay.The mechanical strengths of clay are obtained by direct shear test and unconfined compressive test.Addi-tionally,the acid washing method is employed to obtain the production of calcium carbonate.The enhancement of the mechanical strength of reinforced clay is compared with that of their reference counterpart which uses clear water in-stead of MICP.The results show that the method can effectively reinforce clay,and the shear strength and unconfined compressive strength of the samples are obviously higher than that of their reference counterparts.The mechanical strength of clay with bacterial-binder ratio equal to 1 is the highest(with the unconfined compressive strength reached 192.49 kPa and the production of calcium carbonate reached 4.28%)among the three groups of experiments conduc-ted,followed by the ratio equal to 2.The large amount of calcium carbonate produced corresponds to high improvement of the mechanical strength of clay.The bacterial-binder ratio could affect the production of calcium carbonate and thus affect the mechanical strength of clay.These results demonstrate that the ratio has a significant impact on the effective-ness of reinforcing clay,indicating the importance of optimizing the ratio to improve the effectiveness of MICP.