首页|Collaborative exercise:analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform

Collaborative exercise:analysis of age estimation using a QIAGEN protocol and the PyroMark Q48 platform

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator.Several molecular biomarkers are available for the estimation of chronological age,and currently,DNA methylation patterns are the most promising.In this study,a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories.The assay comprised bisulfite treatment of the extracted DNA,amplification of five CpG loci(in the genes of ELOVL2,C1orf132,TRIM59,KLF14,and FHL2),and sequencing of the amplicons using the PyroMark Q48 platform.Blood samples from 49 individuals with ages ranging from 18 to 64 years as well as negative and methylation controls were analyzed.An existing age estimation model was applied to display a mean absolute deviation of 3.62 years within the reference data set.

forensic geneticsage estimationDNA methylationpyrosequencingtrace sample

Marie-Louise Kampmann、Jan Fleckhaus、Claus B?rsting、Helena Jurtikova、Alice Piters、Julien Papin、Quentin Gauthier、Mirna Ghemrawi、Christian Doutremepuich、Bruce McCord、Peter M.Schneider、Jiri Drabek、Niels Morling

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Section of Forensic Genetics,Department of Forensic Medicine,Faculty of Health and Medical Sciences,University of Copenhagen Copenhagen,Denmark

Institute of Legal Medicine,Faculty of Medicine and University Clinic,University of Cologne,Cologne,Germany

Institute of Molecular and Translational Medicine,Faculty of Medicine and Dentistry,Palacky University Olomouc and the University Hospital Olomouc,Olomouc,the Czech Republic

Laboratoire d'Hématologie Médico-Légale,Bordeaux Cedex,France

Department of Chemistry and Biochemistry,Florida International University,Miami,FL,USA

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work performed in Palacky University,Czech RepublicNational Institute of Justice,Department of Justice,USADepartment of Chemistry and Biochemistry,Florida International University,Miami,FL,USA

LM20181252017-NE-BX-0001

2024

10.1093/fsr/owad055

法庭科学研究(英文)
司法鉴定科学研究院

法庭科学研究(英文)

ISSN：2096-1790
年,卷(期)：2024.9(1)
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