首页|基于磁固相萃取-自动前处理分离和富集动物肝脏中的β-受体激动剂

基于磁固相萃取-自动前处理分离和富集动物肝脏中的β-受体激动剂

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基于磁固相萃取-自动前处理分离和富集动物肝脏中的β-受体激动剂,建立了动物肝脏中3种β-受体激动剂(沙丁胺醇、克伦特罗和莱克多巴胺)残留的液相色谱-串联质谱(LC-MS/MS)分析方法.样品溶液经提取后,采用磁性阳离子吸附剂(M-MCX)结合自动前处理装置进行净化,使用LC-MS/MS检测,同位素内标法进行定量.结果显示,M-MCX较传统混合阳离子固相萃取(MCX)柱的吸附容量高34%,而且节约了吸附材料.结合自动磁固相萃取装置,30 min内可完成8个样品的净化,方法检出限为0.01～0.1 μg/kg,平均回收率为88.2%～110.5%,相对标准偏差为2.9%～10.3%.与传统柱填充式固相萃取法相比,本方法具有操作简单、快速、高效等优点,能够满足动物组织样品前处理的批量、自动化处理的需求.
Separation and Enrichment of β-Agonists from Animal Livers Based on Magnetic Solid-Phase Extraction with Automated-treatment Device
A liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for determination of three kinds of β-agonists(Clenbuterol(CL),Ractopamine(RAC)and Salbutamol(SAL))residues in animal liver samples.The liver sample homogenates were extracted with organic solvent,followed by clean-up using the automatic magnetic solid-phase extraction(MSPE),and then analyzed using LC-MS/MS.The results showed that the magnetic mixed-mode cation exchange adsorbent(M-MCX)exhibited 34%higher adsorption capacity than the conventional mixed-mode cation exchange(MCX)column.Furthermore,the clean-up was conducted by using an automatic MSPE device,and 8 samples could be simultaneously treated within 30 min.The limits of detection(LOD)were 0.01-0.1 μg/kg,the average recoveries ranged from 88.2%to 110.5%,and the relative standard deviations(RSDs)were in range of 2.9%-10.3%at three spiked levels for the three kinds of β-agonists.Compared with the traditional SPE technique,the present method had many advantages such as simple operation,rapidity and high efficiency,which was suitable for high-throughput and automatic detection of residues in routine analysis.

Magnetic solid-phase extractionAutomatic pretreatmentβ-AgonistLiverMass spectrometry

魏书林、王紫豪、李桐、朱怀恩、单吉浩、宋志超、王瑞国

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中国农业科学院农业质量标准与检测技术研究所,北京 100086

普敦实验室设备(上海)有限公司,上海 201108

中国农业科学院农产品加工研究所,北京 100193

河南省兽药饲料监察所,郑州 450015

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磁固相萃取 自动前处理 β-受体激动剂 肝脏 质谱

北京市科技计划项目中国农业科学院创新工程项目国家现代农业产业技术体系项目Innovation Program of the Chinese Academy of Agricultural Science(Feed Quality and Safety)

Z211100007021001CARS-35

2024

10.19756/j.issn.0253-3820.221336

分析化学
中国化学会 中国科学院长春应用化学研究所

分析化学

CSTPCD北大核心
影响因子：1.423
ISSN：0253-3820
年,卷(期)：2024.52(2)
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