Detection of Glucose and Glutathione Based on Nanozymatic and Photoelectrochemical Activities of Au@SH-MIL-101(Fe)
With the rapid development of nanotechnology,the preparation of multifunctional nanomaterials has become a hot research direction in recent years.In particular,gold nanoparticles(AuNPs)are multifunctional,but they are easy to agglomerate into larger particles,affecting their catalytic performance.Using metal-organic framework(MOF)as carriers to suppress the aggregation of AuNPs to obtain multifunctional nanocomposites is a very promising strategy.In this work,a facile and rapid method for loading AuNPs onto surface of SH-MIL-101(Fe)to prepare Au@SH-MIL-101(Fe)nanocomposites was developed.The obtained Au@SH-MIL-101(Fe)exhibited excellent peroxidase(POD)-like activity and the photoelectrochemical(PEC)activity.The structure and chemical composition of Au@SH-MIL-101(Fe)were characterized by powder X-ray diffraction(PXRD),scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS)and Fourier transform infrared spectroscopy(FT-IR),respectively.Based on the POD-like activity,Au@SH-MIL-101(Fe)could catalyze hydrogen peroxide(H2O2)into produce hydroxyl radicals(·OH)to oxidize 3,3',5,5'-tetramethylbenzidine(TMB)at neutral pH to obtain blue oxidation product OXTMB.Therefore,a rapid and sensitive colorimetric method for detection of glucose was established based on the glucose oxidase catalyzing glucose to produce H2O2.The linear range for detection of glucose was 1.0-200.0 μmol/L,and the detection limit(S/N=3)was 0.64 μmol/L.The colorimetric method was used to detect glucose in serum,and the recoveries were 91.7%-106.6%.In addition,Au@SH-MIL-101(Fe)could form an Au—S bond with the sulfhydryl group in GSH to produce a steric hindrance,thereby reducing the photocurrent of Au@SH-MIL-101(Fe).A novel PEC sensor for detection of GSH was constructed by using the superb PEC activity of Au@SH-MIL-101(Fe).The linear detection range of GSH by PEC strategy was 1.0-50.0 nmol/L,and the detection limit(S/N=3)was 0.20 nmol/L.The PEC method was used to detect GSH in serum,and the recoveries was 95.0%-104.5%.The prepared Au@SH-MIL-101(Fe)nanocomposites might provide new insights into the synthesis of innovative multifunctional nanomaterials and promising candidates for clinical applications.
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