首页|基于靶向质谱代谢组学的广陈皮年份鉴别研究

基于靶向质谱代谢组学的广陈皮年份鉴别研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
广陈皮的药用价值随着陈化年份增加而逐渐提高,其价格也随之上涨,导致市场上广陈皮年份欺诈问题严重.针对此问题,本研究选取陈化年份为7～17年的广陈皮作为研究对象,采用基于靶向质谱代谢组学的方法进行年份鉴别.首先,以《中国药典》规定的橙皮苷、橘皮素和川陈皮素等质量评价指标为靶向分析物,与已有研究结果进行对比,评估样品的真实性;然后,利用靶向质谱代谢组学方法计算广陈皮中6种黄酮类化合物的绝对含量以及63种黄酮类化合物和94种黄酮类与非黄酮类化合物的相对含量,结合正交偏最小二乘法判别分析(OPLS-DA),筛选广陈皮中与年份相关的标志代谢物,用以鉴别广陈皮年份.研究结果表明,仅依赖于中国药典规定的3个质量评价指标在内的黄酮类代谢物无法有效鉴别广陈皮的陈化年份,而通过靶向质谱代谢组学方法成功地从广陈皮的94种黄酮类和非黄酮类化合物中筛选得到32种潜在的年份代谢标志物,其中包括荭草素和诺米林等化合物.这些标志物的发现为区分不同年份的广陈皮提供了参考,有望应用于广陈皮的年份鉴别.
Vintage Identification of Guang Citri Reticulatae Pericarpium Based on Targeted Mass Spectrometry-based Metabolomics
The medicinal value of Guang Citri Reticulatae Pericarpium gradually increases with the increase of storage years,leading to a corresponding rise in its market price.This has resulted in a serious issue of fraudulent misrepresentation of vintage identification in the Guang Citri Reticulatae Pericarpium market.To address this issue,the present study focused on Guang Citri Reticulatae Pericarpium aged from 7 to 17 years,employing a targeted mass spectrometry-based metabolomics approach for vintage identification.Initially,this study targeted the analysis of quality evaluation indicators,such as hesperidin,naringin,and neohesperidin,as specified in Chinese Pharmacopoeia.A comparative analysis with existing results was conducted to assess the authenticity of the samples.Subsequently,the absolute content of 6 kinds of flavonoids and the relative content of 63 kinds of flavonoids,along with 94 kinds of flavonoid and non-flavonoid compounds,in Guang Citri Reticulatae Pericarpium were calculated using targeted mass spectrometry-based metabolomics.By integrating orthogonal partial least squares-discriminant analysis(OPLS-DA),the potential metabolites associated with the aging process in Guang Citri Reticulatae Pericarpium were identified.The results revealed that relying solely on the three quality evaluation indicators specified in the pharmacopoeia for flavonoids was insufficient to effectively distinguish the aging process of Guang Citri Reticulatae Pericarpium.However,through targeted mass spectrometry-based metabolomics,the potential age-related metabolites in Guang Citri Reticulatae Pericarpium were successfully identified.Thirty-two kinds of potential vintage metabolic markers,including compounds such as hesperetin and nobiletin,were selected from a pool of 94 kinds of flavonoid and non-flavonoid compounds.The discovery of these metabolites provided an effective means to differentiate Guang Citri Reticulatae Pericarpium of different ages and held promise for potential applications in the authentication of the aging process in Guang Citri Reticulatae Pericarpium.

Guang Citri Reticulatae PericarpiumVintage identificationTargeted mass spectrometry-based metabolomicsVintage metabolic markersOrthogonal partial least squares-discriminant analysis

简晓君、李翰祥、文倩、陶移文、黄洪波、丁博

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广州医科大学药学院,广东省分子靶标与临床药理学重点实验室,广州 511436

广东省药品检验所,国家药品监督管理局药品快速检验重点实验室,广州 510663

中国科学院华南植物园公共实验室,广州 510650

广陈皮 年份鉴别 靶向质谱代谢组学 年份标志代谢物 正交偏最小二乘法判别分析

广东省药品检验所国家药品监督管理局药品快速检验重点实验室开放基金广东省本科高等学校教学质量与教学改革工程建设项目(2022)

KF2022003YJGH[2023]4

2024

10.19756/j.issn.0253-3820.231253

分析化学
中国化学会 中国科学院长春应用化学研究所

分析化学

CSTPCD北大核心
影响因子：1.423
ISSN：0253-3820
年,卷(期)：2024.52(3)
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