Analysis of Hemostatic Properties and Biocompatibility of Cassava Porous Starch-based Hemostatic Powder
Enzymatically modified starch offers a potential biodegradable hemostatic agent.In this study,enzymatic cassava porous starch(ECS)was obtained through hydrolysis using α-amylase and glucoamylase.Subsequently,a cassava porous starch-based hemostatic powder(ECS-Ca-T)was prepared by loading calcium ions(Ca2+)and tannic acid onto the ECS material.The microstructure,pore size distribution,crystal structure,and composition of ECS and ECS-Ca-T were analyzed using scanning electron microscopy(SEM),Fourier transform infrared(FTIR)spectroscopy,X-ray diffraction(XRD),and a specific surface area analyzer(BET).The biocompatibility of ECS-Ca-T was assessed through hemolysis experiments and wound healing experiments.Furthermore,the hemostatic performance of ECS-Ca-T was evaluated through in vitro coagulation tests and rat trauma hemostasis experiments.The biodegradability of ECS-Ca-T was investigated through in vitro degradation experiments.The results indicated that ECS-Ca-T exhibited excellent hemostatic effects,biocompatibility and biodegradability.Compared to commercially available granular hemostatic powder,ECS-Ca-T demonstrated advantages in the hemostasis of persistent bleeding and liver injury in rats,with a shortened hemostasis time of 4.86 min and 8.6 s,respectively.Additionally,ECS-Ca-T enabled rapid hemostasis of rat back muscle tissue within 5 s.Importantly,during the wound healing process,ECS-Ca-T did not cause adverse effects on the body and could degrade up to 94.5%within 7 days.
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