首页|螺旋通道中稳定增强的二次流构建及其粒子聚焦研究

螺旋通道中稳定增强的二次流构建及其粒子聚焦研究

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  • 国家科技期刊平台
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  • NSTL
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惯性微流是一种具有高通量精确操控粒子和细胞能力的微流控技术,近年来受到广泛关注.然而,由于惯性微流芯片内二次流的不稳定性,在大尺寸管道内实现粒子流速不敏感的聚焦仍存在挑战.本研究开发了一种微结构辅助的超低高宽比螺旋微通道,利用二次流的稳定和加速实现了粒子的惯性聚焦.研究结果表明,在大的流速灌注范围(0.5～3.0 mL/min)内成功实现了粒径分别为7.3和15.5 μm的荧光粒子在1mm宽的螺旋通道芯片内的聚焦,聚焦效率分别达到94%和99%以上.此外,粒子聚焦位置距离管道内壁高达100 μm,远大于其它惯性聚焦芯片.因此,通过在螺旋通道芯片内设置等序列的微结构,促使二次流的稳定和增强,完成了流量和粒径不敏感的惯性聚焦.与传统的惯性聚焦方法相比,本方法无需额外的鞘流操作,具有高通量和易于制造的特点.这种创新的结构为便携式惯性微流控芯片的开发提供了更多的选择,可应用于细胞分析与检测、流式细胞仪和在线样品处理等领域.
Spiral Microfluidic for Particle Focusing by Stabilization and Acceleration of Secondary Flow
Inertial microfluidics,as a microfluidic technology with the ability to precisely manipulate particles and cells with high throughput,has attracted widespread attention.However,challenges remain in achieving particle focusing with insensitivity to flow rates in large-scale channels,mainly due to the instability of secondary flows within the inertial microfluidic chip.This study developed a microstructure-assisted ultra-low aspect ratio spiral microchannel,which utilized the stability and acceleration of secondary flows to achieve inertial particle focusing.The research results demonstrated successful particle focusing within a 1 mm-wide spiral channel chip,for different diameter sizes(7.3 μm and 15.5 μm),within a wide range of flow rates(0.5-3 mL/min).The focusing efficiencies for these particles were measured to be above 94%and 99%,respectively.Additionally,it was observed that the particle focusing position was approximately 100 μm away from the channel walls,significantly larger than other inertial focusing chips.Consequently,by incorporating ordered microstructures within the spiral channel chip,the stability and enhancement of secondary flows were achieved,resulting in flow rate and particle size-insensitive inertial focusing.Compared to traditional methods of inertial focusing,this design had advantages of not requiring additional sheath flow operations,and boasted high throughput and ease of manufacturing.This innovative structure opened up vast prospects for the development of portable inertial microfluidic chips,and could be used in the fields such as cell analysis and detection,flow cytometry,and online sample processing.

Microfluidic chipInertial microfluidicsSpiral channelSecondary flowParticle focusing

白汉洁、林智慧、郭时超、龙丹丹、牛颜冰、赵磊、申少斐

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山西农业大学生命科学学院,中兽医药现代化山西省重点实验室,太原 030000

西安电子科技大学生命科学技术学院,西安 710126

微流控芯片 惯性微流 螺旋通道 二次流 粒子聚焦

国家自然科学基金山西省研究生科研创新项目山西省基础研究计划山西省基础研究计划山西省高等学校大学生创新创业训练计划山西省高等学校大学生创新创业训练计划山西农业大学科技创新基金山西农业大学科技创新基金

823721432023KY31820210302123368201801D221251202201732023019920142-112016ZZ08

2024

10.19756/j.issn.0253-3820.231393

分析化学
中国化学会 中国科学院长春应用化学研究所

分析化学

CSTPCD北大核心
影响因子：1.423
ISSN：0253-3820
年,卷(期)：2024.52(4)
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