NaYF4:Yb3+,Er3+@SiO2荧光纳米传感器检测牛奶中过氧化氢
Preparation of a Fluorescent Nanosensor Based on NaYF4∶Yb3+,Er3+@SiO2 for Detection of Hydrogen Peroxide in Milk
彭孔浩 1彭微 1白安琪 1王凌男 1赵伟馨 1武玥 1郭文 1李淑荣 2罗利霞 2孟佩俊2
作者信息
- 1. 包头医学院公共卫生学院,包头 014040
- 2. 包头医学院公共卫生学院,包头 014040;内蒙古自治区卫生检测与评价工程技术中心,包头 014040
- 折叠
摘要
采用溶剂热法合成了稀土掺杂上转换纳米材料NaYF4:Yb3+,Er3+,使用反相微乳液法对其表面进行SiO2包覆制得NaYF4:Yb3+,Er3+@SiO2.采用透射电镜、X射线衍射和傅里叶红外光谱对此材料进行了表征,并基于NaYF4:Yb3+,Er3+@SiO2 与方酸铁(SQA-Fe3+)之间的荧光猝灭原理构建了NaYF4:Yb3+,Er3+@SiO2-SQA-Fe3+荧光纳米传感器,用于微量过氧化氢(H2O2)的检测.在最佳实验条件下,本方法检测H2O2 的线性范围为 1.8～84.0 μmol/L,检出限(3σ)为0.47 μmol/L,牛奶样品中H2O2 的加标回收率为98.4%～99.7%.本方法具有检出限低、稳定性好以及抗干扰能力强等优点,可用于牛奶样品中H2O2 残留的检测.
Abstract
The rare-earth-elements-doped upconversion nanoparticles NaYF4:Yb3+,Er3+were synthesized by solvothermal method,and NaYF4:Yb3+,Er3+@SiO2 were prepared by coating SiO2 on the surface of NaYF4:Yb3+,Er3+by inverse microemulsion method in this work.Based on the fluorescence quenching principle between NaYF4∶Yb3+,Er3+@SiO2 and SQA-Fe3+,a NaYF4∶Yb3+,Er3+@SiO2-SQA-Fe3+fluorescence nanosensor was constructed for detection of trace hydrogen peroxide(H2O2).Under optimal conditions,the linear range of this method for detecting H2O2 was 1.8‒84.0 μmol/L,with detection limit(3σ)of 0.47 μmol/L.The recoveries of H2O2 spiked in milk were 98.4%‒99.7%.This method could be used for detection of H2O2 residue in milk samples,with advantages such as low detection limit,good stability and strong anti-interference ability.
关键词
稀土掺杂上转换纳米材料/过氧化氢/荧光纳米传感器/荧光猝灭Key words
Rare-earth-elements-doped upconversion nanoparticles/Hydrogen peroxide/Fluorescent nanosensor/Fluorescence quenching引用本文复制引用
基金项目
国家自然科学基金(81960601)
国家自然科学基金(82360656)
内蒙古自治区高等学校青年科技英才支持计划(NJYT23026)
内蒙古自治区自然科学基金(2023MS08009)
内蒙古自治区自然科学基金(2021MS08055)
内蒙古自治区卫生健康委医疗卫生科技计划(202201383)
包头医学院研究生科研创新项目(bycx2023006)
自治区级大学生创新创业训练计划(2023)(S202310130001)
出版年
2024
国家科技期刊平台
NSTL
万方数据