首页|基于预涂纳米碳-十二烷基硫酸钠复合基质的基质辅助激光解吸电离质谱分析与猪脑组织切片质谱成像

基于预涂纳米碳-十二烷基硫酸钠复合基质的基质辅助激光解吸电离质谱分析与猪脑组织切片质谱成像

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  • 国家科技期刊平台
  • NETL
  • NSTL
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纳米材料基质对基质辅助激光解吸电离质谱(MALDI-MS)的小分子分析具有独特优势.本研究在比较不同表面活性剂的基础上,建立了预涂纳米碳-十二烷基硫酸钠(SDS)复合基质的方法,避免了纳米碳颗粒作为基质使用时易团聚的问题.实验结果表明,纳米碳-SDS复合预涂基质能够有效抑制纳米基质背景离子峰,显著提高待测成分质谱峰的强度和信噪比.在优化的条件下分析胆固醇、1,2-二棕榈酰-sn-甘油-3-磷酰胆碱(DPPC)和三油酸甘油酯标准品,MS峰强度的点内重复性(RSD)≤5%,点间重复性≤7%.在0.05～1.0 mg/mL范围内测定环磷腺苷和DPPC两种样品时,峰强度与浓度的线性相关系数R2>0.993,显示出较好的定量分析潜力.将纳米碳-SDS复合预涂基质用于猪脑组织的MALDI-MS成像,观察到了猪脑白质和脑灰质中的成分分布差异,显示纳米碳-SDS复合预涂基质具有可用于MALDI-MS成像的潜力.
Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Analysis and Imaging of Porcine Brain Tissue Section Using Precoated Nanocarbon-Sodium Dodecyl Sulfate Composite Matrix
Nanomaterial matrices have unique advantages in small molecule analysis by matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS).On the basis of comparing different surfactants,a method for pre-coating nanocarbon sodium dodecyl sulfate(SDS)composite matrix was established,which could alleviate the issue of aggregation of nanocarbon particles as a matrix.The experimental results showed that the nanocarbon-SDS composite pre-coated matrix effectively suppressed the background ion peak of the nano matrix,significantly improved the MS peak intensity and signal-to-noise ratio of the tested substance.Under the optimal conditions,cholesterol,1,2-dipalmitoyl-sn-glyceryl-3-phosphorylcholine(DPPC),and triglyceride standards were analyzed,with intra-point repeatability(RSD)of MS peak intensity≤5%and inter-point repeatability≤7%.As to measurement of cyclic adenosine monophosphate and DPPC samples in the range of 0.05‒1.0 mg/mL,linear correlation coefficient(R2)between peak intensity and concentration was greater than 0.993,indicating good quantitative analysis potential.The nanocarbon-SDS composite pre-coating matrix was used for MALDI-MS imaging of pig brain tissue,and the differences in component distribution between pig brain white matter and gray matter were observed,demonstrating the potential of nanocarbon-SDS composite pre-coating matrix for MALDI-MS imaging.

Matrix assisted laser desorption ionization mass spectrometryNanocarbon matrixSodium dodecyl sulfateTissue sectionMass spectrometry imaging

庄思佳、沈钰琳、杨帆、龚灿、许旭

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上海应用技术大学化学与环境工程学院,上海 201418

基质辅助激光解吸电离质谱 纳米碳基质 十二烷基硫酸钠 组织切片 质谱成像

国家自然科学基金项目上海应用技术大学科技发展基金项目

31671928KJFZ2023-11

2024

10.19756/j.issn.0253-3820.241085

分析化学
中国化学会 中国科学院长春应用化学研究所

分析化学

CSTPCD北大核心
影响因子：1.423
ISSN：0253-3820
年,卷(期)：2024.52(7)
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