纳米酶增强免疫层析法定量检测血液中的降钙素原
Quantitative Detection of Procalcitonin in Blood by Nanozyme-based Lateral Flow Immunoassay
郑玥 1林童 1熊勇华 2许梦硕 1严喜鸾 3郭徐静 1杨磊 1郭亮2
作者信息
- 1. 南昌大学,食品科学与资源挖掘全国重点实验室,南昌 330047
- 2. 南昌大学,食品科学与资源挖掘全国重点实验室,南昌 330047;南昌大学中德联合研究院,南昌 330047
- 3. 南昌大学药学院,南昌 330006
- 折叠
摘要
建立了一种以金、铂纳米粒子修饰的金属有机框架材料(MAP)为标记物的降钙素原(Procalcitonin,PCT)快速定量免疫层析检测方法.本方法以静电吸附法制备的MAP-抗PCT单克隆抗体偶联物为检测探针,将抗PCT多克隆抗体和羊抗鼠IgG分别喷涂硝酸纤维素(NC)膜构建试纸条的检测线和质控线,采用基于信号放大的夹心免疫法定量检测PCT.结果表明,MAP免疫层析试纸条具有灵敏度高、特异性强和稳定性好等优点,利用其检测PCT的动态检测范围为0.61 pg/mL～320 ng/mL,检出限为0.25 pg/mL,日内和日间精密度(RSD)均小于15%.实际样品检测结果表明,本方法仅需微量样本即可检测全血中PCT的浓度,对于炎症反应的早期诊断、监测治疗及预后判断具有重要意义.
Abstract
A rapid quantitative immunochromatographic assay for procalcitonin(PCT)using metal-organic frameworks modified with gold and platinum nanoparticles(MAPs)as labels was established in this work.The detection probe was prepared by conjugating MAPs with anti-PCT monoclonal antibody via an electrostatic adsorption method.Anti-PCT polyclonal antibody and sheep anti-mouse IgG were sprayed onto the nitrocellulose(NC)membrane as the test line and quality control line,respectively,to construct immunochromatographic strip for PCT quantitative detection via signal-amplification-based sandwich immunoassay.The results showed that the MAP-based immunochromatographic test had high sensitivity,high specificity,and good stability.The dynamic range for detection of PCT was 0.61 pg/mL-320 ng/mL,the detection limit was 0.25 pg/mL,and the intra-day and inter-day precision(Relative standard deviation)were less than 15%.The results of real sample analysis showed that a quite low volume of sample was required for detection of PCT in whole blood,which was of great significance for the early diagnosis,monitoring and treatment,and prognosis of inflammation.
关键词
侧流免疫层析法/降钙素原/快速检测/纳米酶Key words
Lateral flow immunoassay/Procalcitonin/Rapid detection/Nanozyme引用本文复制引用
基金项目
江西省自然科学基金(20232BAB205087)
国家自然科学基金(82360857)
出版年
2024
国家科技期刊平台
NSTL
万方数据