Colorimetric Detection of Sarcosine Based on Functionalized Bimetallic Organic Framework Nanozyme
The functionalized bimetallic organic framework nanozyme (NiFe2-NMOFs) was prepared using solvothermal method for detection of sarcosine content in human urine. The experimental result showed that Fe and Ni were evenly distributed in the material,and Fe existed in the form of Fe2+/Fe3+,Ni existed in the form of Ni2+. Fe2+/Fe3+was the catalytic active center and Ni2+was the adsorption site. The matching of the two elements improved the catalytic activity of the reaction. The successful introduction of amino group induced defects in the structure,formed open accessible sites,and increased water solubility of the material. The NiFe2-NMOFs were proved to have peroxidase-like activity,and the Michaelis-Menten kinetics of NiFe2-NMOFs showd that the material had good kinetic performance and stability. Based on the fact that sarcosine could generate H2O2 under the catalysis of sarcosinase,and the sensing ability of NiFe2-NMOFs to H2O2,a cascade catalytic sensing system of NiFe2-NMOFs-sarcosine oxidase was constructed for detection of sarcosine content in human urine. This sensing system was used to detect sarcosine within the concentration range of 0.2-120μmol/L,with a good linear relationship,and a low detection limit of 0.17μmol/L. This sensing system showed high recoveries (94.1％-99.1％) and lower relative standard deviations (RSDs<5％) when used to detect actual urine samples. As a comparison to the natural peroxidase,this sensing system had better optimum temperature range and other advantages,showing good application prospects in bioanalysis.
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