Study of the Enzyme-free Glucose Biosensor Based on Poly (N-methylaniline)/Ni2+ Modified Glassy Carbon Electrode
At first,the poly(N-methylaniline) (PNMA)film was formed on the glassy carbon electrode by cyclic voltammetric method in 0. 50 moL ? L-1 HCIO4 solution. Then, Ni2+ ions were incorporated by immersion of the modified electrode in a 1. 0 mol?L-1 Ni(NO3)2solution. Thus,a poly(N-methylaniline) /nickel modified glassy carbon electrode was obtained. The electrochemical characteristics and the electrocatalytic activity for glucose oxidation of this modified electrode were investigated. The results showed that PNMA / Ni2+ / GC electrode exhibited high electrochemical activity towards the oxidation of glucose in 0.1 mol?L-1 NaOH solution. At an applied potential of +0. 50 V,it gave a fast response time(2 s) and exhibited a linear response to glucose over the range from 0. 025~5. 70 mmol?L-1 (R = 0. 9996). The detection limit of glucose was 0. 01 mmol?L-1. The standard addition recoveries of glucose were 98%~104%. The interference caused by the interfering species,such as ascorbic acid and uric acid,could be effectively avoided by using Nafion. This electrode is a fairly good enzyme-free glucose biosensor.
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