Determination of Lignin Content and Lignin Monomer Composition in Salvia miltiorrhiza Bge
An accurate, rapid method for determining lignin content and lignin monomer composition in Salvia miltiorrhiza Bge. was established. Acid-insoluble lignin(Klason lignin) and acid-soluble lignin in roots and stems of S. miltiorrhiza were measured by Klason method and UV spectrophotometry, respectively. The lignin monomer in roots and stems of S. miltiorrhiza was extracted by a streamlined thioacidolysis procedure and analyzed via gas chromatography-mass spectrometry(GC-MS), Total lignin content in roots and stems of S. miltiorrhiza were 280. 7 mg/g and 160, 4 mg/g,in which the Klason lignin content was 173. 8 mg/g and 134. 5 mg/g and acid-soluble lignin content was 106. 9 mg/g and 25, 9 mg/g,respectively. The main p-hydroxyphenyl ( H), guaiacyl (G), and syringyl(S) lignin monomer content were 14. 4 μmol/g.2 070, 2 /μmol/g and 1 886. 4 μmol/g in roots,and 15. 3 μmol/g,212. 8 μmol/ g and 50. 6 μmol/g in stems,respectively. The results showed that lignin and its monomer lignin content could be accurately and quickly determined by the method used in this study,and this method might provide reference for the qualitative and quantitative analysis of lignin composition in other medicinal plants.
Salvia miltiorrhiza Bge.Ligninp-Hydroxyphenyl ligninGuaiacyl ligninSyringyl lignin
NETL
NSTL
万方数据
维普
