Double Aptamer-Based Magnetic Bead-SERS-Tagged Sandwich Structure for Rapid Detection of SARS-CoV-2 Virus S Protein
In the face of the extremely rapid spread of coronavirus disease 2019,it is necessary to develop a rapid,accurate and low-cost diagnostic method to detect specific antigens of SARS-CoV-2 virus.Reverse transcriptase polymerase chain reaction(RT-PCR)-based COVID-19 testing requires specific laboratories and is time-consuming.In this study,we proposed a SERS detection platform for SARS-CoV-2 spike protein based on aptamer specific recognition.This procedure allowed simultaneous capture of the substrate and detection probe.SARS-CoV-2 virus and its variants were detected by portable Raman spectrometer within 5 minutes without pretreatment using simulated virus.The experimental results showed that the detection limit of the simulated virus was 200 TU/mL.In addition,the assay could be used for at least five additional variants of SARS-CoV-2 virus,with a limit line of 5 000 TU/mL in the throat swab system.The results of the actual throat swab experiment could be achieved 100%.Therefore,this platform has great application potential in point-of-care rapid diagnosis of SARS-CoV-2.