Synthesizing Core/shell Structured Upconversion Luminescence Nanoparticles for Cytochrome C Detection
In this work,core-shell-shell upconversion luminescence nanoparticles(UCNPs)are synthesized,with an inert core,a confined emitter layer,and an inert shell on the emitter layer enhancing the upconversion luminescence intensity.The as-prepared upconversion nanoparticles possess uniform particle size and good dispersion,and the thickness of the emitter layer and the inert shell are about 2.4 and 2.9 nm,respectively.By modification of the surface of the nanoparticles with the aptamer cytochrome C and its complementary chain,with BHQ3 labelled at the 3'end of the aptamer,the nanoparticles could be applied to quench the luminescence of the materials at 655 nm.In the presence of cytochrome C,the aptamer binds to cytochrome C and detaches from the surface of upconversion nanoparticles,resulting in recovery of emission at 655 nm.In this assay,the emission intensity at 540 nm does not change and can be used as a ratiometric luminescence detection of cytochrome C.The results show that the recovery of luminescence has a good linear relationship with the concentration of cytochrome C in the range of 5 to 80 μmol/L(R2=0.998),and the detection limit is 1 μmol/L.The method provides a new protocol for the luminescence detection of cytochrome C.
Upconversion nanoparticlesCytochrome CLuminescence resonance energy transfer
万方数据
维普
