首页|柱前荧光衍生化法检测降尘样品中的不饱和醛

柱前荧光衍生化法检测降尘样品中的不饱和醛

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本研究发展了一种柱前荧光衍生化方法,实现了重要环境污染物不饱和醛类化合物的高灵敏度、高选择性分析.该方法使用4-丁基羟氨-7-羟基香豆素(HAHC)作为荧光衍生试剂,结合高效液相色谱-荧光检测法(HPLC-FLD)实现了 6种二烯醛的定性、定量分析.在实际样品分析中成功从积尘样品中检测出(E,E)-2,4-庚二烯醛、(E,E)-2,4-辛二烯醛、(E,E)-2,4-十一烷二烯醛.这种柱前衍生化法具有动态范围宽(2～2 500 nmol/L)、灵敏高(检出限为0.2 nmol/L)、精密度好(日内相对标准偏差为2.17％～3.33％,日间相对标准偏差为2.28％～5.04％)等优点,且无需固相萃取等复杂前处理步骤,为痕量醛类污染物检测提供了有效手段.
Detection of Unsaturated Aldehydes in Dust Samples Based on a Pre-Column Derivatization Strategy
Unsaturated aldehydes are a kind of important environmental pollutants.In this study,a pre-column derivatization strategy was developed combined with a liquid chromatography-fluorescence detection method(HPLC-FLD)using 4-butylhydroxylamine-7-hydroxycoumarin(HAHC)as a fluorescent derivatization reagent.After the optimization of the derivatization conditions,a precolumn derivatization method was developed and applied for the analysis of six unsaturated aldehydes with LODs as low as 0.2 nmol/L.In the real sample analysis,(E,E)-2,4-Heptadienal,(E,E)-2,4-Octadienal,(E,E)-2,4-Undecadienal were detected with concentrations in the range of 12.70-136.87 μmol/L.The method has a wide dynamic range(2-2 500 nmol/L)and good precision(intraday RSDs 2.17％-3.33％,interday RSDs 2.28％-5.04％)without complex pretreatment steps,providing a method for the monitoring of unsaturated aldehyde pollutants in various fields.

Nitrone formation derivatization methodUnsaturated aldehydeHigh performance liquid chromatography-fluorescence detection method4-Hydroxyaminobutyl-7-methoxy-coumarin

黄露杰、徐静、汪耀、吕敏、崔玉洁、何振宇、林亚维

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武汉理工大学化学化工与生命科学学院,湖北武汉 430070

武汉市疾病预防控制中心,湖北武汉 430060

硝酮化衍生法 不饱和醛 高效液相色谱-荧光检测法 4-羟氨基丁基-7-甲氧基-香豆素

湖北省卫健委预防专项重大项目武汉预防医学科研专项

WJ2019H303WY19M01

2024

10.13526/j.issn.1006-6144.2024.02.005

分析科学学报
武汉大学,北京大学,南京大学

分析科学学报

CSTPCD北大核心
影响因子：0.717
ISSN：1006-6144
年,卷(期)：2024.40(2)
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