Synthesis and Application of Benzothiazole-based Hypochlorous Acid Fluorescent Probe
A highly selective hypochlorous acid fluorescent probe of HADM was prepared by aldehyde amine condensation of benzothiazole derivatives and diaminomaleonitrile.The HADM probe showed rapid and specific recognition of HClO(reaction time＜3 s)with good pH stability under the interference of reactive oxygen species and reactive nitrogen species,and a clear green fluorescence could be observed.Under the optimized conditions,the fluorescence intensity of the HADM probe showed a good linear relationship with the concentration of hypochlorous acid(R2=0.9969),and the detection limit was 2.64 × 10-6 mol/L.IR,NMR,HRMS and fluorescence spectroscopy studies showed that HC=N bond on the probe was oxidized to form an aldehyde group in the presence of HC1O,resulting in generation green fluorescence.In addition,the probe was successfully applied to naked-eye identifiable HC1O test strips and spiked recovery experiments of complex water samples.With the advantages of simple preparation,strong stability,good specificity,and fast response,the probe can be used as an effective tool for hypochlorous acid detection in practical applications.
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