Determination of Alternaria Toxins in Flower Substitute Tea by Full-automatic QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
A method for the simultaneous determination of 5 kinds of alternaria toxins in flower substitute tea was established by ultra performance liquid chromatography-tandem mass spectrometry combined with full-automatic QuEChERS extraction.The samples were pre-treated by full-automatic QuEChERS experiment instrument,separated by ACQUITY UPLC HSS T3 column and detected by MS/MS system under negative electro-spray ionization(ESI)mode with multiple reaction ion monitoring(MRM).Under the optimized conditions,all the 5 kinds of alternaria toxins were well separated with good linear relationships within their respective linear ranges,and the correlation coefficients(r)were greater than 0.999.The average recoveries of 5 kinds of alternaria toxins in 6 flower substitute tea matrices at 3 kinds of spiked levels ranged from 82.3％to 105.7％,with relative standard deviations no more than 10％,and the detection limits were between 0.5-1.5 pg/kg.The method has characteristics of fast analysis speed,high sensitivity and accuracy,and it can be used for the rapid detection of alternaria toxins in flower substitute tea.
Ultra-high-performance liquid chromatography-tandem mass spectrometryFlower substitute teaAlternaria toxinsFull-automatic QuEChERS
万方数据
维普
