Identification and Determination of Peptide in Earthworm Preparations by LC-MS/MS and Peptiomics
A new method was established for the identification of peptides in the Polypeptide Earthworm Protein Tablet Candy by LC-MS/MS peptiomics and the quantification of peptides by HPLC.In this study,LC-MS/MS was combined with De novo to isolate and preliminatively identify the peptide components in the Polypeptide Earthworm Protein Tablet Candy.The peptide was prepared by chemical synthesis method,and the content of peptide was further verified and determined by HPLC.A total of 482 peptide sequences were identified,and 10 peptides with high relative strength and score were chemically synthesized.The peptide with amino acid sequence LAPVNKPGRF(LF-10)was successfully verified in the actual sample of the Polypeptide Earthworm Protein Tablet Candy.The content of LF-10 was 0.00182%.The combination of LC-MS/MS and De novo provided strong support for the identification and analysis of the polypeptide components of Gylonin.The established LF-10 peptide quantitative analysis method was simple,accurate and reliable,which was helpful for the quality control of the Polypeptide Earthworm Protein Tablet Candy and the further study of Gylonin polypeptide.