Homogeneous Analysis Method of Anti-dsDNA Antibody Based on Photon Burst Counting Technique
In this paper,a novel homogeneous analysis method for anti-dsDNA antibody was reported based on confocal scattering photon burst counting technique and utilizing the strong resonance light scattering properties of gold nanoparticles(GNPs).The experimental conditions such as laser intensity,the number of the bound double-stranded DNA per GNPs,probe concentration and reaction time were optimized and the quantitative analysis method for anti-dsDNA antibody was established.Under optimal conditions,the linear range of the method is 1.0X10-8 mol/L-1.0X 10-7 mol/L,and the detection limit is 5.2 X 10-9 mol/L.Compared with the existing methods,this method has the advantages of high sensitivity,specificity,good reproducibility,fastness and simplicity,and has a broad application prospect in clinical diagnosis and life science.
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