首页|基于代谢组学方法的红花治疗急性酒精性肝损伤的作用机制研究

基于代谢组学方法的红花治疗急性酒精性肝损伤的作用机制研究

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红花在临床上对急性酒精性肝损伤(Acute Alcoholic Liver Inj ury,AALI)有很好的疗效,但治疗机制不甚明确.因此,阐明红花治疗AALI的分子作用机制对药物的进一步开发及应用具有十分重要的意义.实验以雄性Wistar大鼠为研究对象,模型组大鼠灌胃8 mL/kg的酒精以建立AALI模型,给药组大鼠以4.29 g/kg/天的剂量灌胃红花提取物.利用大鼠血清代谢组学分析方法鉴定与AALI相关的潜在生物标志物,并研究红花对这些生物标志物的调控机制.从苏木精和伊红(H&E)染色实验发现,灌胃后红花提取物减轻了肝细胞的损伤程度;与模型组相比,红花组中的丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)的表达水平降低,表明红花提取物具有肝保护作用.通过分析火山图,共鉴定出30种与AALI相关的生物标志物.在这些生物标志物中,红花可以对21个标志物产生回调效果.研究发现,红花可能通过对脂肪酸、磷脂、胆汁酸、氨基酸、核苷酸代谢的调控作用从而对AALI产生治疗效果.本文为红花的推广和临床应用提供了理论基础.
The Therapeutic Mechanism of Flos Carthami in the Treatment of Acute Alcoholic Liver Injury Based on Metabonomics Method
Flos Carthami(FC)has a good therapeutic effect on acute alcoholic liver injury(AALI)in clinical practice,but the treatment mechanism is not very clear.Therefore,elucidating the molecular mechanism of action of FC in treating AALI is of great significance for the further development and application of drugs.The experiment focuses on male Wistar rats as the research object.The model group rats were gavaged with 8 mL/kg alcohol to establish an AALI model,while the administration group rats were gavaged with FC extract at a dose of 4.29 g/kg/day.Potential biomarkers related to AALI were identified using rat serum metabonomics analysis methods,and the regulatory mechanism of FC on these biomarkers was investigated.The H&E staining experiment found that after gavage,FC extract reduced the degree of liver cell damage;Compared with the model group,the expression levels of ALT and AST in the FC group decreased,indicating that FC extract has liver protective effects.Through volcanic map analysis,a total of thirty biomarkers related to AALI were identified.Among these biomarkers,FC can have a callback effect on 21 biomarkers.Research has found that FC may exert therapeutic effects on AALI by regulating the metabolism of fatty acids,phospholipids,bile acids,amino acids,and nucleotides.This article provides a theoretical foundation for the promotion and clinical application of FC.

Flos CarthamiAcute alcoholic liver injuryMetabonomicsUHPLC-MS

王曦烨、阿都沁夫、韩晓静、姜明洋、白梅荣

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内蒙古民族大学化学与材料学院,天然产物化学与功能分子合成自治区重点实验室,内蒙古通辽 028000

内蒙古民族大学护理学院,内蒙古通辽 028000

内蒙古民族大学蒙医药学院,蒙医药研发工程教育部重点实验室,内蒙古通辽 028000

内蒙古民族大学计算机科学与技术学院,内蒙古通辽 028000

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红花 急性酒精性肝损伤 代谢组学 超高效液相色谱-质谱

国家自然科学基金地区项目国家自然科学基金地区项目内蒙古自治区高等学校青年科技英才支持计划内蒙古自治区直属高校基本科研业务费项目内蒙古自治区高等学校创新团队发展计划内蒙古自然科学基金内蒙古自治区高等学校科学研究项目蒙医药研发工程教育部重点实验室开放基金内蒙古民族大学博士启动基金

8226084462162049NJYT23136GXKY22118NMGIRT22162021MS08072NJZY21430MDK2021041BS633

2024

10.13526/j.issn.1006-6144.2024.04.001

分析科学学报
武汉大学,北京大学,南京大学

分析科学学报

CSTPCD北大核心
影响因子：0.717
ISSN：1006-6144
年,卷(期)：2024.40(4)