Distribution of Endogenous Peptides in Different Tissues Extracted from Bighead Carp based on Mass Spectrometry
Trace endogenous peptides exist widely in organisms as functional bioactive substances,which are usually closely related to physiological activities such as cell growth,substance metabolism,and immune genetics,and play an important regulatory role.Due to the different physiological functions of different tissues and organs,the types and quantities of endogenous peptides are obviously different.In this work,the endogenous peptides in different tissues of Bighead carp were studied by mass spectrometry,and their species and distribution characteristics were discussed.Based on magnetic solid phase extraction(MSPE),polymer-modified magnetic nanomaterials Fe3O4@SiO2@PAA and Fe3O4@PEI were used for selective enrichment of endogenous peptides in samples.The differences of endogenous peptides in blood and different organs were compared and analyzed by proteomics method,and their potential physiological functions were revealed by gene analysis.The results showed that the number of endogenous peptides in fish brain was the highest,while the number in fish gut was the lowest.The results of mass spectrometry also showed that the endogenous peptides in different tissues and blood of bighead carp were quit different,and there were commonalities and individual differences in their types and functions,and the different endogenous peptides were related to the physiological structure and function of the organs.The results of this study initially revealed the relationship between the physiological functions and biological processes of endogenous peptides in different tissues of fish,and provided a theoretical basis for further studies on spatial proteomics,the function of different tissues and the molecular mechanism of virus invasion into cells.
Bighead carpEndogenous peptidesSeparation and enrichmentProteomics
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