Simultaneous Determination of 14 Indicative Components from Five Plant Extracts in Cosmetics by UPLC-MS/MS
A method of ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was developed for simultaneous determination of 14 indicative components from 5 plant extracts in cosmetics.The sample was ultrasonically extracted with 80％methanol solution,then separated on a Phenomenex Kinetex F5 column(100 mm×3.0 mm,2.6 μm)by gradient elution using 0.1％formic acid solution and acetonitrile as the mobile phases.The mass spectrometry was used with electrospray ionization source,positive and negative ion simultaneous scanning and multiple reaction monitoring mode.The results showed that there were good linear relationships for 14 indicative components in their respective mass concentration rangers with the correlation coefficients(r)larger than 0.99.The limits of detection were in the ranges of 0.1-19.0 μg/kg and the limits of quantitation were in the ranges of 0.3-53.3 μg/kg.The recoveries at three spiked levels for 14 indicative components in four types of matrix were 83.6％-119.0％with the relative standard deviations of 1.1％-9.7％(n=6).With the advantages of simple,fast and high sensitivity,this method is suitable for qualitative identification and quantitative analysis of indicative components of multiple plant extracts in cosmetics.
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