首页|MC-LR磁性分子印迹微球结合高效液相色谱-串联质谱对鱼肉中微囊藻毒素的测定

MC-LR磁性分子印迹微球结合高效液相色谱-串联质谱对鱼肉中微囊藻毒素的测定

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建立了 MC-LR磁性分子印迹微球(Fe3O4@MIP)结合高效液相色谱串联质谱法(HPLC-MS/MS)测定鱼肉中的8种微囊藻毒素(MCs).以MC-LR为模板,甲基丙烯酸(MAA)为功能单体,在磁性纳米颗粒外包裹SiO2,在其表面合成对MCs有特异性识别的MC-LRFe3O4@MIP.并对其磁性、吸附性能和特异性进行检测.将MC-LR Fe3O4@MIP作为磁性固相萃取的吸附剂,在优化条件下测定鱼肉中8种MCs,该方法在0.1～10 μg/L的范围内呈现良好的线性关系(R2≥0.99),检出限均为0.05 μg/kg,定量限均为0.15 μg/kg,平均回收率在74.8％～92.7％之间,相对标准偏差在1.1％～4.5％之间.结果表明,利用MC-LR Fe3O4@MIP能实现鱼肉中MCs的绿色、高效、特异性检测.
Determination of Microcystins in Fish by MC-LR Magnetic Molecular Imprinted Microspheres Combined with High Performance Liquid Chromatography-Tandem Mass Spectrometry
A method of MC-LR magnetic molecularly imprinted microsphere(Fe3O4@MIP)combined with high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)was established to determine 8 types of microcystins(MCs)in fish.MC-LR Fe3O4@MIP with specific recognition for microcystins were synthesized on its surface by using MC-LR as the template,methacrylic acid as the functional monomer,and magnetic nanoparticles encapsulated in silica as the carrier.Its magnetism,adsorption performance,and specificity were tested.And it was used as an adsorbent for dispersed solid phase extraction(DSPE)to determine 8 types of MCs in fish under optimized conditions.The developed method exhibited a good linearity in the range of 0.1 μg/L to 10 μg/L,with correlation coefficients above 0.99.The detection limits(LODs)for all 8 types of MCs were 0.05 μg/kg and the limits of quantification(LOQs)were 0.15 μg/kg.The mean recoveries were within 74.8％-92.7％,with a relative standard deviation(RSD)of 1.1％-4.5％.The results indicate that using MC-LR Fe3O4@MIP can achieve green,efficient,and specific detection of MCs in fish.

MC-LR magnetic molecular imprinting microspheres(MC-LR Fe3O4@MIP)FishMicrocystinsHigh-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS)

徐潇颖、李帆、刘柱、梁晶晶

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国家市场监管重点实验室(功能食品质量与安全)浙江省市场监管保健食品质量安全重点实验室浙江省食品药品检验研究院,浙江杭州 310052

磁性分子印迹微球(Fe3O4@MIP) 鱼肉 微囊藻毒素 高效液相色谱串联质谱

浙江省公益技术研究计划(分析测试)项目浙江省市场监督管理局检验监测技术装备项目浙江省市场监督管理局科技项目浙江省市场监督管理局雏鹰计划核心项目

LGC21C200001ZB202246620210129CY2022227

2024

10.13526/j.issn.1006-6144.2024.04.009

分析科学学报
武汉大学,北京大学,南京大学

分析科学学报

CSTPCD北大核心
影响因子：0.717
ISSN：1006-6144
年,卷(期)：2024.40(4)