Determination of Toluene Diamine in Urine by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry
An ultra high performance liquid chromatography-tandem mass spectrometry method was established for the determination of 2,6-TDA and 2,4-TDA in urine.The urine sample was extracted by solid-phase extraction after hydrolysis,cleaned up with acetonitrile and separated on a Waters HSS T3 chromatography column.The analysis was carried out under positive ionization mode(ESI+)and multiple reaction monitoring(MRM)conditions,and the contents of 2,6-TDA and 2,4-TDA in urine were quantitatively determined by the external standard method.The results showed that the determination of 2,6-TDA and 2,4-TDA in urine achieved good linearity in the range of 0.039-10 ng/mL and 0.098-10 ng/mL,with a correlation coefficient(r)greater than 0.999.The limits of detection and the limits of quantification were 0.012 ng/mL,0.029 ng/mL and 0.039 ng/mL and 0.098 ng/mL,respectively.The recovery in spiked urine ranged from 94.65％to 102.42％.The intra batch and inter batch precisions were 1.62％-6.67％and 2.85％-4.10％,respectively.This method can be used for monitoring and evaluating urinary 2,4-TDA and 2,6-TDA in the general population and occupational exposure to toluene diisocyanate.
Urine2,4-Tolylenediamine(2,4-TDA)2,6-Tolylenediamine(2,6-TDA)Ultra-high performance liquid chromatography tandem mass spectrometry
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