Construction of Molecularly Imprinted Double Emission Ratio Fluorescence Sensor and the Sensitive Detection of Microcystin
This article focuses on the study of microcystin(MCs)in water.A dual emission ratio fluorescence sensor was constructed to detect the content of MCs in water via the prepared B-CDs and N-CDs with using metformin as a virtual template for molecular imprinting.A cavity structure similar to common MCs(MC-LR,-RR,-YR)was formed on the surface of silica microspheres through imprinting and elution because of metformin and microcystin having the same guanidine group.After recombining with MCs,the B-CDs fluorescence in the polymer would respond to MCs,producing significant fluorescence quenching,while the N-CDs fluorescence would not respond to MCs.Therefore,the ratio fluorescence analysis of MCs was achieved with B-CDs fluorescence using as a target sensitive signal and N-CDs fluorescence using as a reference signal.The experimental results show that the constructed sensor in this paper has good selectivity and high sensitivity to MCs.The sensor has a linear relationship between 0.5 and 150 μg/L,with a linear equation of I=0.00375c+1.03019,a correlation coefficient of R2 of 0.9870,and a detection limit of 0.025 μg/L.This sensor can effectively detect MCs and has great development prospects for the detection of algal toxins in actual samples.
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