Combination of Pipette Tip Solid Phase Extraction and High Performance Liquid Chromatography for Determination of Quercetin in Food Samples based on the Metal-Organic Framework/Graphene Oxide as Highly Efficient Sorbent
In this study,Cu-based metal-organic framework/graphene oxide(Cu-MOF/GO)nanocomposite was used as a sorbent for the pipette tip micro-solid phase extraction(PT-μSPE)of quercetin from food samples.The metal-organic framework/graphene oxide composites were prepared by hydrothermal method.The composites were characterized by Fourier transform infrared spectroscopy,X-ray diffraction and scanning electron microscopy.The adsorption parameters including solution pH,flowrate of sample,adsorbent amount,volume of sample,elution solvent and its volume were optimized by batch adsorption experiment.Twenty mg adsorption material could adsorb 96％ of quercetin in samples(pH 6.0)within 25 min,and 1 mL 0.1％ methanoic acid-methanol could fully desorb the target.Under the optimized extraction conditions,there was a good linearity in the range of 0.1-120 μg/L quercetin with a limits of detection of 0.066 μg/L.The average recoveries for quercetin were between 87.4％ and 109.5％ with RSD of 1.7％-4.9％ in spiked food samples.The saturated adsorption capacity of the adsorption material was 196.0 mg/g,which could be reused for many times.The results showed that the prepared adsorbent provided desirable adsorption capacity of quercetin,demonstrating great potential in the detection of quercetin in complex food matrices.
万方数据
维普
