Study on the Application of Aluminon in the Determination of Chloramphenicol by Resonance Light Scattering Spectrometry
Resonance light scattering spectroscopy is widely used in the field of drug analysis due to its high sensitivity,simple operation,and low cost.In this experiment,the aluminon-chloramphenicol supermolecule system was constructed through hydrogen bond interaction,and the quantitative relationship between resonance light scattering intensity and the concentration of chloramphenicol was established,so as to realize the content determination of chloramphenicol.The influence of experimental conditions was investigated in detail,and the results showed that the concentration of aluminon was 5.0×10-4mol/L,the volume was 1.5 mL,the dosage of hydrochloric acid was 2.5 mL,and the concentration and volume of chloramphenicol were 100 μg/mL and 4.0 mL,the resonance light scattering of supermolecule system was the most weakened,and the molar ratio of chloramphenicol and aluminon was 2∶1(mol/mol).This method exhibited good linearity in the concentration range of 8-40 μg/mL(y=156.26x+3919.6,R2=0.9906),with a detection limit of 0.01420 μg/mL.The accuracy of chloramphenicol at the spiked samples of 8,10,12 μg/mL was evaluated,with average recoveries of 100.6%,100.1% and 100.6%,and RSD% of 1.07%、0.68% and 0.75%,respectively(n=5).Finally,the method was successfully applied to the content determination of chloramphenicol eye drops,and the average content was 100.1%,RSD%was 0.74%(n=6).The above results showed that the resonance light scattering spectrometry established could be used for the quantitative analysis of chloramphenicol.