Application of a Fluorescent Probe Based on Excited-State Intramolecular Proton Transfer and Aggregation-Induced Emission Properties for the Detection of Cu(Ⅱ)and Glutathione
A diphenylaniline-salicylic acid Schiff base compound(PDSA)had been synthesized.The molecule exhibits obvious aggregation-induced emission(AIE)and excited state intramolecular proton transfer(ESIPT)property and a large Stokes shift(135 nmol/L).A highly sensitive and selective fluorescence sensing method for Cu2+and glutathione(GSH)was designed.The fluorescence of the molecule at 555 nm was quenched with the addition of Cu2+.When the concentration of Cu2+ranged from 0.2 μmol/L to 1.0 μmol/L,the fluorescence intensity showed a good linear relationship with the concentration of Cu2+,and the detection limit was 14 nmol/L.PDSA and Cu2+(PDSA/Cu2+)can be combined as a composite probe for the fluorescence enhancement detection of glutathione,and the composite probe has high selectivity and sensitivity.The composite probe has good linearity in the GSH concentration ranged from 1.0 μmol/L to 14.0 μmol/L,and the detection limit was about 179 nmol/L.In addition,the method also showed good test results in real samples.
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