Determination of 19 Sulfonamides and 2 Sulfonamide Synergist Residues in Aquatic Products by HPLC-MS/MS
A high-performance liquid chromatography-tandem mass spectrometry method(HPLC-MS/MS)with solid phase extraction was established for the simultaneous determination of 19 sulfonamides residues and 2 synergists in fisheries products using isotope internal standard for quantification.The effects of different pre-treatment conditions,purification conditions,and chromatographic conditions on the experimental results were investigated.The samples were extracted with acetonitrile,purified by MCX solid-phase extraction column and determined by LC-MS/MS selective multiple reaction monitoring with positive ion mode for qualitative and quantitative analysis.The results showed that the detection limits and quantification limits of 21 sulfonamides were 1.0 μg/kg and 2.0 μg/kg,respectively.The relative standard deviations were between 2.45％-13.9％(n=6)and the recoveries were between 67.8％-115％.This method was validated by using four substrates,including carp,shrimp,sea cucumber,and turtle.It has the characteristics of wide applicability,high sensitivity,and good repeatability,and can be applied to the residual determination of various sulfonamide and their synergist in aquatic products.
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