A Rapid Screening Method Based on High Resolution Orbit-Trap Mass Spectrometry for Detection of Fornitrofuran Metabolites in Aquatic Products
A rapid screening method for detection of four kinds of nitrofuran metabolites in aquatic products was established by ultra high performance liquid chromatography-quadrupole/orbital trap high resolution mass spectrometry,and a mass spectrum database was established to realize high throughput screening without standard substances.Nitrofuran metabolites were derivatized at 50℃ and separated by Hypersil GOLD column.Furazolidone,furfuratone,furantoin and furacillin were detected by Full MS/dd MS2.The linear range was 1-100 ng/mL,the correlation coefficients were greater than 0.99,the detection limits were 0.5 μg/kg,the recoveries were 62.5％-113.3％,and the relative standard deviations were 2.6％-13.5％.The pretreatment is rapid,sensitive and reproducible,and could be used for the rapid screening of poppy husk alkaloids in food.
Nitrofuran metabolitesHigh resolution mass spectrumSquatic products
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