In order to increase the profile of signal-noise ratio of pulsar signals from surface observation,a de-noising method based on alterable moving window and two-tiered 3σ-rule is proposed in this paper.According to alterable moving window in this method,pulsar signals are processed step by step continuously to avoid missing noise.Using two-tiered 3σ-rule to filter noise can prevent excessive de-noising.The observed data of J1939-+ 2134 and J1744-1134 by the Jiamusi 66 m telescope at Jiamusi deep space station is used for validation.The result shows that this method can promote signal-noise ratio obviously and remove the noise effectively.