Research on the fermentation technology of recombinant SOD expression in Pichia pastoris
In this article,study fermentation conditions of the recombinant strains in shake culture,aiming at improving the protein expression and fermenting in 5 L fermentation tank.The result indicates that the suitable fermentation conditions for the recombinant cell are as below:the temperature of cell growth and protein expression is 30 ℃,the methanol feeding is 2％ (volume ratio of the medium)per day,the initial pH is 6.2,the induction time is 72 h.According to these conditions,the enzyme activity of shake culture is increased by 64％ comparing with before optimization.In 5 L fermentation cultivate,the enzyme activity reaches 40 864 U · mL-1,86 times of the shake culture.
国家科技期刊平台
NSTL
万方数据
维普
