In order to study effective extracting conditions,pigment components extracted from naturally brown cotton with an ethanol/water solution system were investigated.The ratio of ethanol to water solution had significant influence on the pigment components extracted from naturally brown cotton.It is showed that the components with a retention time of 1-5 rain was easily separated,while those with the retention time of 7-17 min was hard to be separated.The pigments in naturally brow cotton are strongly hydrophilic and extracting efficiency of the pigments is very low if over 90％ethanol/water solution is used,which is not suitable for the pigment extraction.While 0％ethanol solution was suitable for extraction of components with the retention time of 1.4 min,30％ethanol solution was suitable for extraction of components with the retention time of 1.7 min,2.2 min and 9.6 min,and 50％ethanol solution was suitable for extraction of components with the retention time of 14.6 min.
naturally brown cottonpigment of brown cottonethanol/water solutionhigh performance liquid chromatography
NETL
NSTL
维普
万方数据
