In order to develop tissue regeneration materials with certain electrical conductivity, we prepared silk fibroin nanofiber membranes using electrospinning and further polypyrrole/silk fibroin conductive nanofiber membranes by in-situ oxidative polymerization. The effect of spinning parameters on the surface morphology of nanofiber membrane was studied. The electrical conductivity of nanofiber membrane was tested by the four-point probe. FT-IR spectrometer was used to characterize the chemical structure of nanofiber membrane. The results showed that silk fibroin nanofiber membranes have an even surface with less beads, with an average fiber diameter of ( 520. 70 ± 140. 81 ) nm, when solution concentration was 0. 16 g/mL, flow rate 0. 2 mL/h, voltage 20 kV, rotating speed 1 000 r/min. Polypyrrole/silk fibroin conductive nanofiber membranes was prepared by in-situ oxidative polymerization, and it retains its original nano fiber structure, and the electrical conductivity is ( 0. 44 ± 0. 07) S/cm, when the pyrrole concentration was 0. 3 mol/L, dopant concentration 0. 3 mol/L, the ratio of fixed pyrrole to oxidant 1∶2, and the polymerization time 6 h.
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