Aiming at the understanding of reduction of bending stiffness and energy dissipation in ultra-fine metal yarns after cyclic loading, yarns made from gold coated molybdenum wires with different structures were studied subject to 50 cycles of bending and 200 cycles of reciprocating friction, followed by the comparison of the morphology, tensile properties and electrical properties. The mechanical and structural responses of the yarns under cyclic bending load and friction reciprocating load were investigated, and the knittability of the yarns from metal wires was quantitatively analyzed. The results show that the micro-and macro-structures of gold coated molybdenum wire are damaged, the mechanical properties are decreased and the contact electrical resistance is slightly increased under the cyclic bending and reciprocating friction loads. The tensile rigidity and strength of double-stranded gold coated molybdenum yarns are greatly decreased under the same conditions, which is not suitable for fabric knitting. The dynamic mechanical properties and electrical properties of mono- and triple-stranded gold-coated molybdenum wires are stable under dynamic load, which can be used as raw materials for knitting electromagnetic shielding fabrics.