Fabrication of antibacterial polymers coated cotton fabrics with I2 release for wound healing
Objective Using raw cotton fabrics as medical cotton dressings in wound treatment cannot effectively prevent wound infection.Cotton dressings with antibacterial function can effectively prevent wound infections,but most of the antibacterial agents used for antibacterial finishing on cotton fabric face issues of resistance and high toxicity.In order to enhance the value of medical cotton-based dressings by ensuring safe and effective disinfection of wounds on wound management,a safe and low toxicity preparation strategy is urgently needed to endow cotton materials with excellent bactericidal ability.Method Iodophor has been widely used for wound disinfection because of its good bactericidal effect and biocompatibility.However,few studies focused on the release of I2 from cotton materials to prevent wound infections,mainly due to the low chelation strength of cotton materials to I2,making it difficult to ensure sufficient I2 release.In order to provide safe antibacterial effects to ordinary cotton materials,we selected less toxic I2 as the antibacterial active ingredient.Cotton fabrics were coated with good biocompatibility of sodium carboxymethyl cellulose(CMC)/polyvinyl pyrrolidone(PVP)hybrid polymer,adding coated cotton fabrics into potassium iodide(KI)solution until full adsorption and sufficient swelling of the cotton fabrics.After that,hydrogen peroxide with a concentration of 3％was added to oxidized KI into I2.Finally,through impregnation method,complexed I2 molecules were attached onto the surface of the coated cotton fabric.Results Both CMC and PVP are hydrophilic polymers,and the cotton fabric coated with CMC/PVP hybrid polymer still showed good water absorption ability and were able to adsorb I2 up to 18.6 μg/mg,the existence of polyvinyl pyrrolidone made cotton fabric demonstrated a strong I2 adsorption,ensured bactericidal effect against bacteria.Owing to the excellent film-forming properties of sodium carboxymethyl cellulose(CMC),cotton fabrics coated with CMC/PVP polymers formed a smooth surface,and the PVP polymer on the surface of cotton fabrics adsorbed a large amount of I2.The inhibition zones test confirmed that cotton fabric complexing with I2 was able to continuously release I2 to form an antibacterial ring.Owing to the strong oxidation ability of I2,cotton fabric complexing with I2 was able to quickly kill bacteria in contact via destroying bacteria cell membrane.Cyto-toxicity experiments confirmed that all cotton fabrics,whether before or after coating and complexing with I2,exhibited low toxicity against fibroblasts,and the cell survival rate of all samples was above 90％.When cotton fabric was used for bacterial infection wound treatment,the released I2 killed 99.9％of bacteria at the wound tissue,significantly accelerating wound healing speed.In addition,the release of iodine ions effectively reduced the inflammatory response caused by bacterial infections,thereby accelerating wound healing.Conclusion Finishing cotton fabric with CMC/PVP hybrid polymer coating is proven to maintain good water absorption performance and increase the chelating cap ability of cotton fabric to I2,and after complexing with I2 molecules,cotton fabric has almost no toxic effect on cellular tissues.Owing to the adsorption of full dose I2,when using cotton fabrics complexing with I2 for wound infected with bacteria,this cotton fabric can slowly release I2 to kill bacteria at the wound tissues.In summary,using this cotton fabrics releasing I2 can not only prevent wound infections but also reduce the inflammatory response caused by bacterial contamination,thereby accelerating wound healing.This cotton fabric as a medical dressing to treat wounds can effectively prevent wound infection without the need for disinfection.Therefore,this cotton fabric dressing can be a good substitute for current wound dressings and has great potential in the clinical treatment of wound infections.
万方数据
维普
