Preparation and properties of cotton fabric with poly(N-isopropylacrylamide)antibacterial hydrogel
Objective In order to reduce the incidence of chronic wound infections,various antibacterial dressings play an important role in wound treatment,among which silver-based antibacterial hydrogel is advantageous.However,sudden release of silver antibacterial agent and poor mechanical properties of hydrogel are the two factors limit lug the application of silver-based antibacterial hydrogel in wound repair.In order to expand the application of silver-based antibacterial hydrogel and achieve sustained release antibacterial,the construction strategy of hydrogel composite fabric was adopted to prepare a new type of hydrogel composite fabric integrated dressing.Method Combined with the chemical crosslinking and ultra violet light initiation method,the polyazoiso propyl acrylamide(PNIPAM)/silver loaded with graphene oxide(GO-Ag)hydrogel was combined with cotton fabric to form a new kind of dressing with the integration of hydrogel and fabric.The number of cotton fabric layer used for constructing dressing was explored.The structure,antibacterial properties,tensile strength,and biological safety of dressings were discussed by scanning electron microscopy,infrared spectroscopy,fluorescence inverted microscopy,etc.Results Based on research of PNIPAM/GO-Ag composite antibacterial hydrogel,the PNIPAM/GO-Ag composite cotton fabric with different layers was constructed.According to the analysis results of microscopic morphology and molecular structure,the PNIPAM/GO-Ag hydrogel was evenly interpenetrated into cotton fabric because of the strong interface integration.Analyzing the antibacterial,mechanical,and biological properties of PNIPAM/GO-Ag hydrogel composite cotton fabric with different layers,it was found that the integrated dressing,composed of PNIPAM/GO-Ag hydrogel and three-layer cotton fabric,demonstrated excellent comprehensive performance.In contrast with the single cotton fabric,the tensile breaking strength of the integrated dressing were improved by 73.7％and reached 370 N in the wet state.This proved that the integrated structure of hydrogel composite cotton fabric could help strengthen the mechanical property of hydrogel,which is beneficial for the promotion and application of wound dressing.The in vitro cytotoxicity of dressing was 0 level,and the hemolysis rate was less than 5％,demonstrating the PNIPAM/GO-Ag hydrogel composite cotton fabric illustrated good biological safety,providing possibilities for its application in the field of wound repair.The inhibition rate of antibacterial dressing on the Escherichia coli and Staphylococcus aureus reached more than 98％.Compared with the slow-release antibacterial effect of PNIPAM/GO-Ag hydrogel,the PNIPAM/GO-Ag hydrogel composite cotton fabric developed slow-release effect of silver within 24 h,proving that the hydrogel composite fabric has a more durableability to continuously release silver release silver ions,and the slow-release antibacterial effect of dressing was enhanced.In addition,from the analysis of bacterial micro-structure and intracellular reactive oxygen species(ROS)content,it was concluded that the antibacterial effect of hydrogel composite cotton fabric was mainly achieved by continuously releasing silver ions,inducing bacteria to produce ROS and causing oxidative damage to bacteria,thereby destroying bacteria.Conclusion The combination strategy of hydrogel composite fabric is one of the methods to construct a new integrated antibacterial dressing,which has the characteristics of both hydrogel and fabric.It was found that the interface between the hydrogel and cotton fabric directly affects the structural integrity of the overall dressing.The number of cotton fabric layer determines the interface binding force between the hydrogel and each layer of the fabric,and ultimately affects its tensile properties,antibacterial properties,and biological safety.Compared with the single cotton fabric,in the wet state,the tensile breaking strength of PNIPAM/GO-Ag composite cotton fabric integrated dressing has strengthened.And the slow-release antibacterial effect of silver ions in PNIPAM/GO-Ag composite cotton fabric is more pronounced than that of PNIPAM/GO-Ag hydrogel.
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维普
