首页|高分子量壳聚糖皮芯结构微纳米纤维膜制备

高分子量壳聚糖皮芯结构微纳米纤维膜制备

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高分子量壳聚糖因具有良好的抗菌性、可促进细胞和组织生长以及可降解等特性,被认为是促进伤口愈合的优良材料;然而高分子量壳聚糖纺丝液黏度大,用常规静电纺丝法难以制成微纳米纤维.为解决这个问题,采用溶液喷射纺丝法制备了高分子量壳聚糖/聚氧化乙烯微纳米纤维,纺丝液含有质量分数为1.6%的高分子量壳聚糖和2.5%~5.0%的聚氧化乙烯(相对分子质量为10万).所制备的微纳米纤维呈直线形,表面呈不光滑波纹状.通过MatLab对扫描电子显微镜照片进行识别和计算,结果表明,当聚氧化乙烯质量分数从2.5%增加到5.0%时,纤维平均直径从133 nm增加到210 nm,直径分布变宽;微纳米纤维材料中大孔隙增多,孔隙率在0.69左右,变化不大;X射线光电子能谱和透射电子显微镜分析结果表明,所制备的高分子量壳聚糖/聚氧化乙烯喷射纺丝微纳米纤维具有皮芯结构,其中,聚氧化乙烯位于皮层,高分子量壳聚糖构成微纳米纤维的内芯;动物实验初步证明了高分子量壳聚糖皮芯结构微纳米纤维可以促进伤口愈合.
Fabrication of high molecular weight chitosan core-shell nanofibers
Objective High molecular weight chitosan(HMCS)has many advantages when used in the field of wound management because of its antibacterial properties as well as the cell and tissue growth capabilities.However,fabricating HMCS nanofiber is challenging since the spinning solution's viscosity is extremely high.In order to solve this problem,solution blow spinning was studied and adjusted to fabricate HMCS nanofibers,and the spinning parameters were identified to fabricate polyethylene oxide(PEO)as shell and HMCS as core nanofibers,which were transformable to physical hydrogel when contacting the wound exudate for wound healing.Method The spinning solutions containing 1.6%mass fraction HMCS and 2.5%-5.0%mass fraction PEO were prepared by dissolving and mixing these two species in 50%mass fraction aqueous acetic acid solutions,with 200 r/min mixer rotation rate and 10 h mixing time.The well-mixed solutions were degassed for 12 h before solution blow spinning.In the spinning process,PEO-HMCS nanofibers were spun with the parameters ranging from 0.04 MPa to 0.10 MPa air pressures and 21 cm to 33 cm collecting distances.The area of the resulting PEO-HMCS nanofibers was 2 010 cm2 and the spinning duration was 45 min for each of the four spinning solutions.The temperature was kept at 24 ℃ with the relative humidity of approximately 20%during the solution blow spinning process.Results The morphologies of the PEO-HMCS nanofibers were observed by scanning electron microscopy and field emission-scanning electron microscopy.The shapes of the nanofibers were straight lines and the fiber surfaces were not smooth,with some ripple shapes.When the PEO mass fractions in solutions increased from 2.5%to 5.0%,the mean diameters of the nanofibers increased from 133 nm to 210 nm,with the nanofibers porosities anchanged and remaining at 0.69.This study also investigated the influences of changing collecting distances on the resulting nanofibers mean diameters,as well as the influences of changing air pressures on the resulting nanofibers mean diameters.When the collecting distances increased from 21 cm to 27 cm,the PEO-HMCS nanofibers mean diameters decreased first and then increased.As the air pressures increased from 0.04 MPa to 0.05 MPa,the mean diameters increased from 637 nm to 790 nm.After further increasing air pressures to 0.07 MPa,the mean diameters dropped to 375 nm.Continuing increasing the air pressures to 0.10 MPa led to the mean diameters decreasing from 359 nm to 397 nm.The detailed nanofiber core shell structures were observed by the transmission electron microscopy.Before immersed in water,the thickness of the fiber shell was 340 nm and the thickness of the fiber core was 35 nm approximately.After immersed in water,the thickness of the fiber shell significantly decreased.When the PEO mass fraction increased from 2.5%to 5.0%,the mean diameters of the PEO-HMCS nanofibers increased from 161 nm to 211 nm,with conductivities decreasing from 1 760 μS/cm to 1 640 μS/cm and viscosities increasing from 42 082 mPa·s to 91 055 mPa·s.The dynamic viscosities of PEO 2.5%mass fraction solution dropped quickly to 0.11 Pa·s before shear rate reached 1s-1,and remained unchanged afterwards.The dynamic viscosities of 1.6%HMCS solution decreased slowly during shear rate sweeping from 0.1-1 000 s-1,and the values were all higher than those of 2.5%PEO solution.For the dynamic surface tensions,higher PEO mass fractions led to lower dynamic surface tensions.Furthermore,no nitrogen element was detected on the nanofiber surfaces by the X-ray photoelectron spectroscopy.The in vivo animal experiment results showed that the PEO-HMCS nanofibers significantly promoted wound healing.Conclusion The types as well as the mass fractions of HMCS and PEO were studied for fabricating HMCS nanofibers.The nanofibers showed unique morphological structures,mean diameters,and pore distributions.Several specialiZed solution blow spinning parameters,including air pressures and collecting distances,could influence the fabrication process.The solution viscosities,conductivities,and surface tensions also had an impact on the HMCS nanofibers formation.No HMCS was found on the nanofibers surfaces and only PEO existed.The resulting PEO-HMCS solution blow spinning nanofibers had core shell structures,with PEO mainly locating at the shell region and HMCS at the core region.The shell of the nanofiber was semi-flexible and the core was stiff with no flexibility.The in vivo animal experiment results showed that the PEO-HMCS core shell nanofibers could be used as the physical hydrogels to promote wound healing.

medical dressingsolution blow spinningchitosanpolyethylene oxidemicro-nanofibercore-shell structure

房磊、刘秀明、贾娇娇、蔺志浩、任燕飞、侯凯文、巩继贤、扈延龄

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天津工业大学纺织科学与工程学院,天津 300387

青岛大学纺织服装学院,山东青岛 266071

青岛大学附属医院创伤外科,山东青岛 266000

山东欣悦健康科技有限公司,山东滨州 256600

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医用敷料 溶液喷射纺丝法 壳聚糖 聚氧化乙烯 微纳米纤维 皮芯结构

2024

纺织学报
中国纺织工程学会

纺织学报

CSTPCD北大核心
影响因子:0.699
ISSN:0253-9721
年,卷(期):2024.45(9)