Micro-CT and small animal high-frequency ultrasound were used to evaluate experimental rheumatoid arthritis
Objective To establish the evaluation criteria of experimental rheumatoid arthritis by micro-CT and high-frequency ultrasound.Methods Five IL-1β knockout mice with spontaneous arthritis and five SD rats with rheumatoid arthritis(CIA)induced by bovine type Ⅱ collagen were constructed.Then,the toes of arthritis model mice were imaged by micro-CT,and the microscopic parameters such as BV/TV and BS/TV were analyzed.High-frequency ultrasound was used to image the knee joint of arthritis model mice to detect the echo intensity of the knee joint and observe whether there was effusion in the knee joint.Results Micro-CT could clearly detect the enlargement and deformity of the toes in the arthritis model mice,and the microscopic parameters BV/TV and BS/TV were significantly reduced.High-frequency ultrasound could clearly observe the knee joint effusion in arthritis model mice,and the echo intensity of knee joint gray value quantitative response in arthritis model group was significantly lower than that in control group.Conclusion The application of micro-CT and high-frequency ultrasound imaging analysis of small animal toes and knees can systematically evaluate the development of experimental rheumatoid arthritis,and compensate for the lack of unified standards in experimental rheumatoid arthritis imaging.
万方数据
维普
