Alveolar bone marrow mesenchymal stem cells sheet combined with PCL/HA scaffold displays better osteogenesis effect in the early healing process:based on animal models
Objective To investigate the repairing effect of alveolar bone marrow mesenchymal stem cells (Al-BMSCs) sheet combined with PCL/HA scaffold in rabbit mandibular defects. Methods Al-BMSCs sheets and Long bone marrow mesenchymal stem cells (Lon-BMSCs) sheets were cultured, PCL/HA scaffolds were produced by the fused deposition modeling method. Nine New Zealand rabbits were used to establish bilateral mandibular defect models. According to the implant materials, the rabbits were divided into 3 groups:Group A (PCL/HA scaffold), group B (Lon-BMSCs sheets combined with PCL/HA scaffold), group C (Al-BMSCs sheets combined with PCL/HA scaffold). A total of 18 samples were harvested, with 6 samples per group. All the experimental animals were sacrificed after 4 weeks. Gross observation, cone beam CT analysis, HE staining and Masson staining were used to evaluate the effect of bone regeneration. Results The results of cone beam CT showed that the value of BV/TV, Tb.Th, Tb.N in the group C were higher than that in group A and group B, and the difference was statistically significant (P<0.05). HE staining and Masson staining results showed that the osteogenesis in the group C was the most active, characterized by more newly formed bone and capillaries. Conclusion Al-BMSCs sheets combined with PCL/HA scaffold displays better osteogenesis effect in the early healing process, which is expected to become a new strategy for the regeneration of maxillofacial bone defects.
alveolar bone marrow mesenchymal stem cellscell sheetscaffoldbone defect
国家科技期刊平台
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