Experimental study on the therapeutic effect and immune response of Ultrasound-Guided Co-Ablation System in the treatment of Hepa1-6 tumor-bearing mice
Objective To evaluate the therapeutic efficacy and immunological impact of an ultrasound-guided combined cryoablation and thermal ablation system(Co-Ablation System)in the management of Hepa1-6 tumor-bearing mice.Methods A hepatoma mouse model was established using healthy C57BL/6J mice inoculated with Hepa1-6 tumor cells.Forty tumor-bearing mice were randomly assigned to four groups:a blank control group,a sham operation group,a surgical operation group,and a Co-Ablation System group,with ten mice per group.Utilizing ultrasound,tumor volume alterations were assessed pre-treatment and at 1,2,3,and 4 weeks post-treatment.Flow cytometry was employed to quantify the percentages of CD4+and CD8+T lymphocytes in peripheral blood samples.Additionally,three mice from each group were euthanized two weeks post-treatment for histological analysis via HE staining and immunohistochemistry to determine the optical density of CD4+and CD8+cells.Results The tumor tissues in both the blank control group and the sham operation group exhibited gradual growth at each time point,with no statistically significant difference observed between the two groups(P>0.05).In contrast,the tumor volume in the Co-Ablation System group progressively decreased post-treatment,showcasing a statistically significant difference compared to the blank control and sham operation groups(P<0.05).Notably,the serum levels of CD4+T and CD8+T cells in the Co-Ablation System group were markedly elevated compared to the blank control,sham operation,and surgical operation groups at all post-operative time points(P<0.05).These levels peaked three weeks after treatment in the Co-Ablation System group,subsequently declining.Additionally,a significant increase in CD4 and CD8 positive cells within tumor tissue was observed in the Co-Ablation System group two weeks post-treatment,as compared to the blank control and sham operation groups,with the difference being statistically significant(P<0.05).Conclusion The utilization of the Ultrasound-guided Co-Ablation System has proven to be highly effective in treating Hepa1-6 tumor mice,not only reducing tumor burden but also activating the immune system and potentiating the body's anti-tumor immune response.
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