Relationship between IL-1,IL-1β,IL-6 and IL-10 gene polymorphisms and the occurrence of diabetic periodontitis
Objective To analyze the relationship between interleukin-1(IL-1),interleukin-1β(IL-1β),interleukin-6(IL-6)and interleukin-10(IL-10)gene polymorphisms and the occurrence of diabetic periodontitis.Methods 60 patients with diabetic periodontitis admitted to the hospital from June 2021 to June 2022 and 60 healthy people who received oral examination at the same time were defined as the observation group and the control group,respectively.The expression levels of IL-1,IL-1β,IL-6 and IL-10 in serum and gingival crevicular fluid were compared between groups.IL-1,IL-1β,IL-6 and IL-10 gene polymorphisms in whole blood DNA were detected,and their relationship with the susceptibility to diabetic periodontitis was ana-lyzed.Results The levels of IL-1,IL-1β,IL-6 and IL-10 in the serum and gingival crevicular fluid in the ob-servation group were significantly higher than those in the control group(t=31.987,28.911,14.201,16.562,21.315,19.146,-45.554,-57.942,P<0.05).In both groups,the levels of IL-1,IL-1β,IL-6 and IL-10 in the gingival crevicular fluid were significantly higher than those in the serum(t=-4.080,-10.316,-10.686,10.713;t=-9.567,-6.422,-9.904,3.944,P<0.05).In the observation group,IL-1 rs7413228,IL-1β rs2356789,IL-6 rs5357964 and IL-10 rs4543211 were found to be associated with the occurrence of diabetic periodontitis(P<0.05).The frequencies of allele T of IL-1 rs7413228,allele T of IL-1β rs2356789 and allele Gof IL-10 rs4543211 were correlated with the occurrence of diabetic periodontitis(P<0.05).The polymor-phisms of IL-1 rs7413228,IL-1β rs2356789,IL-6 rs5357964 and IL-10 rs4543211 were identified as indepen-dent influencing factors of diabetic periodontitis(P<0.05).Conclusion IL-1,IL-1β,IL-6 and IL-10 gene polymorphisms are associated with susceptibility to diabetic periodontitis.In clinical practice,the risk of diabet-ic periodontitis can be assessed by testing for gene polymorphisms.