Effect of inactivated preservation solution on influenza A virus nucleic acid detection results
Objective To determine the effect of inactivation solution on the detection of influen-za A virus nucleic acid under different storage conditions and time by detecting positive samples from large samples.Methods From February 2022 to March,a total of 2 377 patients with suspected influenza in Shanxi Bethune Hospital were recruited.Two oral swabs were taken from each patient.One of swabs was placed in a tube containing an inactivation solution,another swab is placed in a tube containing a non-inactivation fluid.Specimens were separated into 3 EP tubes.One tube was subjected to nucleic acid detection immediately(with-in 4 hours after collection),and the other two tubes were stored below 2～8℃and-20℃,respectively,and nu-cleic acid detection was performed 48 hours later.Real-time fluorescence reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the viral RNA.When the results were inconsistent,the second nucleic acid detection reagent was used for comparison.The difference of viral load detection was compared be-tween two preservation methods,preservation conditions and preservation time.Result The positive detec-tion rates of influenza A virus in both groups were the same at immediate detection,both of which were 74.5%(1 771/2 377).The average circulating threshold(Ct)value in the non-inactivated group was(29.60±3.99),while in the inactivated group was(31.30±2.67),with no statistically significant difference(t=0.35,P>0.05).There was no significant difference between the two groups after 48 hours of preservation at 2～8℃and-20℃.The mean CT values of the non-inactivation group were(31.70±4.91)and(30.30±4.03),respec-tively,and the inactivated group was(33.90±5.11)and(32.20±4.62),the difference was not significant(t= 0.67,0.54,P>0.05).Conclusion The inactivation sample solution does not affect the results of influenza A virus qRT-PCR.Influenza A virus RNA can be stored for in the inactivated sample preservation solution at 2～8℃or-20℃for at least 48 hours.
Influenza A virusInactivationSample preservation solutionNucleic acid
NETL
NSTL
万方数据
维普
