Clinical significance of the TUBA1C in colon cancer
Objective To investigate the expression of TUBA1C gene in colon cancer,analyze the relationship between expression and prognosis with patients with colon cancer,and explore the relationship between TUBA1C gene and colon cancer.Methods RNA sequencing data of colon cancer tissues and normal tissues were downloaded from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)da-tabase.R 4.3.0 software was used to extract the expression value of TUBA1C mRNA.Then,the expression of TUBA1C in normal tissues and colon cancer were analyzed.In the following analysis,the correlation between TUBA1C expression and clinicopathological parameters of patients were explored.Moreover,the Kaplan-Mei-er survival analysis was utilized to analyze the relationship between TUBA1C expression and overall survival rate of patients.The COX regression analysis was used to explore the prognostic role of TUBA1C in colon can-cer.In addition,GEPIA2 database and UALCAN database were utilized to verify the expression of TUBA1C and its predictive effect on prognosis.Gene set enrichment analysis(GSEA)was used to find the molecular pathways between TUBA1C gene and the occurrence and development of colon cancer.Results Compared with the normal tissues,TUBA1C mRNA was significantly upregulated in colon cancer tissues,the difference was statistically significant(P<0.05).The expression level of TUBA1C was significantly correlated with the tu-mor stage of patients(P<0.05).Moreover,the overall survival rate of patients with high TUBA1C expression was significantly lower than that of patients with the low expression group,the difference was statistically sig-nificant(P<0.05).Multivariate COX regression analysis showed that TUBA1C could be an independent prog-nostic factor for colon cancer patients(HR=1.993,95%CI:1.077～3.010,P<0.05).GSEA enrichment analysis revealed that TUBA1C was involved in cell cycle,DNA replication,mismatch repair,and the P53 signaling pathway in colon cancer.Conclusion The TUBA1C gene is highly expressed in colon cancer tissue and is re-lated to the patients'prognosis.It can participate in the occurrence and development of colon cancer through a variety of carcinogenic pathways,which may become a new molecular marker of colon cancer.
NETL
NSTL
万方数据
维普
