Correlation and clinical significance of PARP1 and ferroptosis in chemotherapy-resistant epithelial ovarian cancer tissues
Objective To investigate the correlation between poly-ADP-ribose polymerase 1(PARP1)and ferroptosis in chemotherapy resistant epithelial ovarian cancer and its clinical significance.Meth-ods A total of 80 patients with epithelial ovarian cancer at Yingshang County People's Hospital in Fuyang City,Anhui Province,from February 2019 to February 2023 were selected for this study.These patients had received platinum-based chemotherapy at least 4 times and were divided into two groups:chemotherapy sensi-tive(n=31)and chemotherapy resistant(n=49)based on their response to treatment.Immunohistochemistry was used to detect the protein expression of PARP1 in epithelial ovarian cancer tissues before chemotherapy.Additionally,fluorescence quantitative PCR was used to measure the mRNA expression of PARP1 and ferrop-tosis marker genes GPX4,SLC7A11 and TfR11 in epithelial ovarian cancer tissues.The levels of PARP1,GPX4,SLC7A11 and TfR1 expression between the two groups were compared.Patients were followed up to assess their progression-free survival(PFS)and overall survival(OS).Results The high expression rate of PARP1 in epithelial ovarian cancer tissues with FIGO stageⅢ～Ⅳand CA125≥35 U/mL was higher than that of epithelial ovarian cancer tissues with FIGO stage Ⅰ～Ⅱand CA125<35 U/mL.The difference was statisti-cally significant(χ2=4.129,9.095,P<0.05).The relative mRNA expression levels of PARP1,GPX4 and SLC7A11 as well as the high expression rate of PARP1 were higher in chemotherapy resistant tissues compared to chemotherapy sensitive tissues.Conversely,the relative mRNA expression level of TfR1 was lower in che-motherapy resistant tissues than in chemotherapy sensitive tissues.The difference was statistically significant(t=23.487,20.030,23.378,22.752,χ2=5.905,P<0.05).The mRNA relative expression level of PARP1 in epi-thelial ovarian cancer was positively correlated with the mRNA relative expression levels of GPX4 and SLC7A11(correlation coefficient 0.351 and 0.394,respectively),and negatively correlated with the mRNA rel-ative expression level of TfR1(correlation coefficient-0.364).Patients with high PARP1 expression in epitheli-al ovarian cancer had shorter OS and PFS compared to patients with low PARP1 expression.The difference was statistically significant(χ2=4.851,5.623,P<0.05).Conclusion The high expression of PARP1 is correlated with chemotherapy resistance,reduced ferroptosis and poor survival prognosis in epithelial ovarian cancer.
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NSTL
万方数据
维普
