Serum levels of miR-211 and miR-128 in cutaneous malignant melanoma and their relationship with efficacy
Objective To explore the levels of serum miR-211 and miR-128 and the relationship with efficacy in cutaneous malignant melanoma(CMM).Methods 120 patients with CMM admitted to the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine from July 2021 to June 2022 were selected as the CMM group,and 90 patients with non-tumor skin diseases during the same period were enrolled as the control group.The expression levels of serum miR-211 and miR-128 were compared between the two groups,and the relationship between expressions of serum miR-211 and miR-128 and clinicopathologi-cal characteristics of CMM was analyzed.After 3 months,the patients in the CMM group were divided into the remission group and the non-remission group according to the efficacy of solid tumors.The relationship be-tween expressions of serum miR-211 and miR-128 and efficacy of patients was analyzed.Results The expres-sion levels of serum miR-211 and miR-128 in the CMM group were lower than those in the control group(t= 18.186,48.871,P<0.05).There was a statistically significant difference in the expression of serum miR-211 among patients with different pathological types,differentiation degrees,lymph node metastasis,clinical stag-es and Ki-67 index(F or t=13.209,3.044,5.601,7.996,3.748,P<0.05).There was a statistical significance in the expression of serum miR-128 among patients with different differentiation degrees,lymph node metasta-sis and clinical stages(t=5.940,9.592,12.895,P<0.05).There were statistical differences in pathological type,differentiation degree,presence or absence of lymph node metastasis,clinical staging,Ki-67 index and presence or absence of surgery between the remission group and the non-remission group(χ2=8.062,19.171,7.333,14.436,5.025,4.309,P<0.05).The expression levels of serum miR-211 and miR-128 in the remission group after treatment were higher compared to the non-remission group(t=13.926,8.849,P<0.05).Multivari-ate Logistic regression analysis showed that poor differentiation(OR=2.275,95%CI:1.027~5.042),lymph node metastasis(OR=2.643,95%CI:1.243~5.622),clinical stageⅢ~Ⅳ(OR=3.022,95%CI:1.348~6.777),low expression of miR-211(OR=2.208,95%CI:1.217~4.006),and low expression of miR-128(OR=2.375,95%CI:1.086~5.192)were risk factors affecting the efficacy of patients(P<0.05).Conclusion The expres-sion levels of serum miR-211 and miR-128 in patients with CMM are significantly decreased and are related to clinicopathological characteristics and efficacy of patients and have certain reference value.