Application and evaluation of the one-step nucleic acid detection method in urogenital pathogen infection
Objective To explore the feasibility of the one-step nucleic acid extraction method for pathogens in urogenital infections and provide an experimental basis for its wide clinical application. Methods Secretion specimens were collected from 200 patients with urogenital infection at the People's Hospital of Shenzhen Baoan District from March to August 2021. Nucleic acid was extracted using both the one-step method and the conventional boiling method to compare the consistency,sensitivity,anti-interference ability and repeatability of the two extraction methods. Results The results showed that the positive consistency between the one-step method and the boiling method had a kappa value of 0.949,indicating good consistency between the two methods. Both extraction methods were able to detect a dilution concentration of up to 1:100000 for the selected positive samples. The interference offsets of the two extraction methods were within 10％. Repeatability experiments demonstrated that the coefficient of variation(CV)values for both extraction methods were within 5％. Conclusion There was no significant difference between the two extraction methods. The one-step method is simple and quick to operate,reducing the risk of laboratory contamination and has great clinical application value.
万方数据
维普
