Combined detection of KRAS,NRAS,BRAF,PIK3CA,and NTRK1 gene expression in colorectal cancer
Objective To investigate the relationship between mutations in KRAS, NRAS, BRAF,PIK3CA,and NTRK1 genes and clinicopathological characteristics in patients with colorectal cancer (CRC),and to explore the prognostic value of combined detection in CRC patients. Methods A total of 200 CRC patients were selected as research subjects ,and the clinical data were collected. DNA was extracted from CRC surgical resection or puncture biopsy specimens,and the mutation status of the KRAS,NRAS,BRAF, PIK3CA,and NTRK1 genes was detected using a human cancer multi-gene mutation combined detection kit. The relationship between the mutation status and pathological features of CRC patients was analyzed. The patients were divided into groups based on whether they had recurrent CRC during follow-up ,and the diagnostic value of single or combined detection of KRAS,NRAS,BRAF,PIK3CA,and NTRK1 gene mutation status for CRC recurrence was analyzed. Additionally,Kaplan-Meier survival curve and log-rank method were used to compare the survival rates of the two groups. Cox regression analysis was conducted to comprehensively analyze the risk factors affecting the prognosis of patients. Results KRAS and BRAF gene mutations were found to be associated with pTNM staging(x2=6.714,5.451,P<0.05),and there was a correlation between KRAS and BRAF gene mutations(r=-0.157,P=0.027),while no correlation was observed between other gene mutations(P>0.05). Sngle gene detection of KRAS,NRAS,BRAF,PIK3CA,and NTRK1 and combined multi-gene detection were found to have certain value in predicting CRC recurrence. The combined detection of KRAS,NRAS, BRAF,PIK3CA,and NTRK1 genes showed the highest diagnostic accuracy for CRC recurrence(AUC=0.797), but the combined detection of KRAS,BRAF,PIK3CA,and NTRK1 had the highest sensitivity(0.861). PIK3CA had the highest specificity(0.969). The overall survival rate and disease-free survival rate at 3 and 5 years in CRC patients without KRAS,NRAS,BRAF,PIK3CA,and NTRK1 gene mutations were higher than those in CRC patients with gene mutations. Cox regression analysis showed that age over 60,mutations in KRAS, BRAF,PIK3CA,and NTRK1 genes were independent predictors of overall survival in CRC patients(P<0.05). Conclusion KRAS,NRAS,BRAF,PIK3CA and NTRK1 gene mutations in CRC patients do not have a significant correlation. However,the detection of each gene,either individually or in combination,holds value in predicting CRC recurrence. Making it an important indicator for evaluating the prognosis of patients.
万方数据
维普
